biotinylated goat anti rabbit igg secondary antibody (Vector Laboratories)

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Vector Laboratories biotinylated goat anti rabbit igg secondary antibody
Biotinylated Goat Anti Rabbit Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 19583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+anti+rabbit+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pmc13059316-74-7-15
Average 96 stars, based on 19583 article reviews

biotinylated goat anti rabbit igg secondary antibody - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: Seq-Scope-eXpanded: spatial omics beyond optical resolution
Article Snippet: For DAB-based immunostaining, the tissues were incubated overnight at 4 °C with primary antibodies diluted in blocking solution, using anti-COL1A1 (72026, Cell Signaling, 1:300), anti-ACTA1 (17521-1-AP, Proteintech, 1:200), and anti-p-4E-BP1 (2855, Cell Signaling, 1:200). .. After primary incubation, the sections were washed three times with Ab-wash solution for 5 minutes at room temperature and then incubated with biotinylated anti-rabbit secondary antibody (BA-1000, Vector laboratories, 1:200) for 40 min at room temperature. .. Following the same washing procedure, the tissues were incubated with Streptavidin-Horseradish Peroxidase (554066, BD Biosciences, 1:300) for 35 min at room temperature.

Article Title: Nitrergic neurotransmission within the lateral hypothalamus modulates cardiovascular responses to stress in rats
Article Snippet: After the blockage, the slices were incubated with anti-nNOS primary antibody (cat. #51973; dilution 1:1000; ABBKine, Atlanta, GA, USA) for 24 h at 4 °C. .. After the incubation, the slices were washed with PBS 3 times (10 min each wash) and incubated with biotinylated anti-rabbit secondary antibody (cat.# BA-1000; dilution 1:600; Vector Laboratories, Burlingame, CA, USA) in PBS-Tx (0.25% Triton X-100) and 3% goat serum for 2 h at room temperature. .. The slices were then washed with PBS 3 times (10 min each wash) and incubated for 1 h in avidin–biotin-peroxidase solution (cat.# PK-6100; Vectastain Elite ABC-HRP Kit, Peroxidase—Standard; Vector Laboratories, Burlingame, CA, USA), 0.5% Triton X-100 and PBS.

Article Title: Age-Dependent Alterations of Chromosomal Passenger Complex Members During Implantation and Decidualization in the Mouse Uterus.
Article Snippet: Tissue sections were incubated with rabbit polyclonal antipIncenp (Affinity Biosciences #AF8485) at 1:100 dilution, rabbit polyclonal anti- Aurora Kinase B (Abcam #ab2254) at 1:100 dilution, rabbit polyclonal anti- Survivin (Cell Signaling #2808S) at 1:50 dilution, rabbit polyclonal anti- Borealin (MyBioSource #MBS127771) at 1:100 dilution, and rabbit polyclonal anti- Ki- 67 (ProteinTech #28074- 1- AP) at 1:1000 dilution, incubated at 4°C overnight. .. Subsequently, the samples were incubated with biotinylated anti- rabbit secondary antibody (dilution: 1:500) (#BA1000, Vector Laboratories, Burlingame, USA) in a humidified environment at 25°C for 45 min. .. The samples were washed with PBS and incubated for 20 min with streptavidin peroxidase (# TS125- HR; Thermo Scientific/Lab Vision).

Article Title: Seq-Scope-eXpanded: spatial omics beyond optical resolution.
Article Snippet: For DAB-based immunostaining, the tissues were incubated overnight at 4°C with primary antibodies diluted in blocking solution, using anti-COL1A1 (72026, Cell Signaling, 1:300), anti-ACTA1 (17521-1- AP, Proteintech, 1:200), and anti-p-4E-BP1 (2855, Cell Signaling, 1:200). .. After primary incubation, the sections were washed three times with Ab-wash solution for 5 minutes at room temperature and then incubated with biotinylated anti-rabbit secondary antibody (BA-1000, Vector laboratories, 1:200) for 40 minutes at room temperature. .. Following the same washing procedure, the tissues were incubated with Streptavidin-Horseradish Peroxidase (554066, BD Biosciences, 1:300) for 35 minutes at room temperature.

Article Title: Nitrergic neurotransmission within the lateral hypothalamus modulates cardiovascular responses to stress in rats.
Article Snippet: After the blockage, the slices were incubated with anti-nNOS primary antibody (cat. #51973; dilution 1:1000; ABBKine, Atlanta, GA, USA) for 24 h at 4 °C. .. After the incubation, the slices were washed with PBS 3 times (10 min each wash) and incubated with biotinylated anti-rabbit secondary antibody (cat.# BA-1000; dilution 1:600; Vector Laboratories, Burlingame, CA, USA) in PBS-Tx (0.25% Triton X-100) and 3% goat serum for 2 h at room temperature. .. The slices were then washed with PBS 3 times (10 min each wash) and incubated for 1 h in avidin–biotin-peroxidase solution (cat.# PK-6100; Vectastain Elite ABC-HRP Kit, Peroxidase—Standard; Vector Laboratories, Burlingame, CA, USA), 0.5% Triton X-100 and PBS.

Avidin-Biotin Assay:

Article Title: Engram and neural underpinning dynamics of the long-lasting childhood olfactory memory
Article Snippet: .. To investigate the neural networks activated in response to the childhood odorant, cFos immunochemistry was performed on brain slices (AP coordinates ranging from +6.05 to −2.59) using cFos antibody (1:2000, Abcam, 214672) followed by a biotinylated anti-rabbit secondary antibody (1/200, Vector Laboratories, BA-1000), then revealed through an avidin-biotin-peroxidase complex (ABC Elite Kit 1/100, Vector Laboratories, PK-6100) and amplified with 3,3-Diaminobenzidine (DAB 0.05%, Sigma-Aldrich, D5905). ..

Amplification:

Immunofluorescence:

Article Title: Collectin-11 regulates osteoclastogenesis and bone maintenance via a complement-dependent mechanism
Article Snippet: A biotinylated secondary antibody was applied for 1 h at RT, followed by ABC kit and DAB (Vector Labs), counterstaining with hematoxylin, and dehydration for mounting with DPX (Sigma). .. For immunofluorescence, a biotinylated anti-rabbit secondary antibody (Vector Labs; BA-1000) was applied for 1 h at RT, followed by FITC-conjugated streptavidin (GeneTex; GTX30950) or streptavidin-594 (Vector Labs; SA-5594). .. Nuclear staining was performed using DAPI (1:10,000 in PBS; Life Technologies) before mounting with PermaFluor (LabVision).

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